Back to Blog
Unsupervised cluster analysis partitioned the cell lines in a manner that reflected their motile/invasive capacity. Western blot analysis for selected proteins confirmed the expression profiles revealed by MudPIT, demonstrating the fidelity of this high-throughput analysis. Within whole cell extracts of the ovarian cancer cells, MudPIT identified proteins that mapped to 2245 unique genes. Six ovarian cancer cell lines were subjected to proteomic characterization using multidimensional protein identification technology (MudPIT), and evaluated for their motile/invasive behavior, so that these parameters could be compared. The aim of this study was to reveal expression profiles associated with the highly motile/invasive ovarian cancer cell phenotype. Proteomic profiling has emerged as a useful tool for identifying tissue alterations in disease states including malignant transformation.
0 Comments
Read More
Leave a Reply. |